ExperimentalOutstanding Survival and Regeneration Process by the Use of Intelligent Acellular Dermal Matrices and Mesenchymal Stem Cells in a Burn Pig Model
Section snippets
Material and Methods
A 15 kilogram, 3-month-old healthy Duroc pig was selected for the experiment. At all times good veterinary and laboratory animal practices were used and pain avoided by intervention of professional personnel. A large, deep 20 cm by 15 cm burn affecting the full thickness of skin with muscle and rib involvement was artificially made in the right lateral thoracic-abdominal wall of the anesthetized pig. We immediately made a deep fascial scarectomy of the burned tissue (Fig 1a). The wound bed was
Results
The animal did not show any sign of suffering or pain before, during, or after the burn and after all other surgical procedures. It was managed at all times under strict veterinary control. At the first observation of the wound on the seventh day after the burn, the animal was in a good general state but with some paradoxical movements of the right hemilateral thoracic and abdominal walls. After removing the external bandages and covering film, a clear integration of the matrices to the wound
Discussion
For many years we have used acellular pig dermal matrices for the treatment of burns and skin defects. These biologic scaffolds are easily obtained and cheap to produce. They are rendered acellular by physical and chemical methods; their availability is unlimited.11, 12, 13 They integrate into the wound bed, gently promoting healing consistently achieving good results. Also, we have already shown that concomitant with extensive and critical burns, MSCs circulate in large amounts. These cells
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