Elsevier

Transplantation Proceedings

Volume 42, Issue 10, December 2010, Pages 4275-4278
Transplantation Proceedings

Experimental
Outstanding Survival and Regeneration Process by the Use of Intelligent Acellular Dermal Matrices and Mesenchymal Stem Cells in a Burn Pig Model

https://doi.org/10.1016/j.transproceed.2010.09.132Get rights and content

Abstract

A pig model with a deep large burn was used to study the regeneration process induced by mesenchymal stem cells (MSCs) and acellular pig dermal matrices, made intelligent by the combination with biodegradable nanofibers loaded with growth factors (granulocite-macrophage colony-stimulating factor and epidermal growth factor) and coated with the anti-CD44 monoclonal antibody (intelligent acellular dermal matrices, IADMs). These IADMs are specially designed to integrate in the wound bed as new biological scaffolds as well as to specifically recruit and attach circulating and/or externally applied MSCs through the anti-CD44 antibody while delivering precise amounts of growth factors. In this way, the reparative process as well as the aesthetic and functional results were enhanced in our burn model. The animal survived, the wound was completely closed, and total regeneration of the skin was obtained without much scarring. Surprisingly, hair follicles and other skin appendages developed despite the severity and deepness of the burn. Even burned muscles and ribs seemed to have undergone a regenerative process by the end of the study. Based on these findings, we have proposed the use of IADMs and autologous, allogeneic or xenogeneic MSCs, as a new paradigm for the future treatment of large burns and probably other dermatological and cosmetic human conditions.

Section snippets

Material and Methods

A 15 kilogram, 3-month-old healthy Duroc pig was selected for the experiment. At all times good veterinary and laboratory animal practices were used and pain avoided by intervention of professional personnel. A large, deep 20 cm by 15 cm burn affecting the full thickness of skin with muscle and rib involvement was artificially made in the right lateral thoracic-abdominal wall of the anesthetized pig. We immediately made a deep fascial scarectomy of the burned tissue (Fig 1a). The wound bed was

Results

The animal did not show any sign of suffering or pain before, during, or after the burn and after all other surgical procedures. It was managed at all times under strict veterinary control. At the first observation of the wound on the seventh day after the burn, the animal was in a good general state but with some paradoxical movements of the right hemilateral thoracic and abdominal walls. After removing the external bandages and covering film, a clear integration of the matrices to the wound

Discussion

For many years we have used acellular pig dermal matrices for the treatment of burns and skin defects. These biologic scaffolds are easily obtained and cheap to produce. They are rendered acellular by physical and chemical methods; their availability is unlimited.11, 12, 13 They integrate into the wound bed, gently promoting healing consistently achieving good results. Also, we have already shown that concomitant with extensive and critical burns, MSCs circulate in large amounts. These cells

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