Bone marrow/stem cell transplantationCorrelation Between the Immature Characteristics of Umbilical Cord Blood–Derived Mesenchymal Stem Cells and Engraftment of Hematopoietic Stem Cells in NOD/SCID Mice
Section snippets
Isolation of CD34+ Cells From UCB
UCB was obtained from the umbilical cords of full-term deliveries after receiving informed consent. UCB collected in bags containing heparin were processed within 24 hours. After separation over Ficoll-Hypaque (Histopaque-1077; Sigma, St Louis, MO), low-density cells were washed in RPMI-1640 (Gibco-Invitrogen, Grand Isle, NY) supplemented with penicillin (20 U/mL; Gibco-Invitrogen), streptomycin (20 μg/mL; Gibco-Invitrogen), and 2% heat-inactivated fetal calf serum. CD34+ cells were isolated
Results
To identify the effects of UCB-MSCs on the homing and engraftment of hHSCs, we intravenously infused both MSCs and CD34+ HSCs into NOD/SCID mice. We successfully isolated UCB-MSCs from various donors and cultured them until passage 4. FACS analysis showed these cells to be positive for typical MSC antigens (CD29, CD44, CD105, CD166), but negative for typical hematopoietic antigens (CD14, CD34, CD45), and HLA-DR (MHC class II), indicating that they were MSCs (Fig 1A, B). Also, expanded UCB-MSCs
Discussion
The major finding of this study was that the UCB-MSCs that were most effective in promoting the engraftment of hHSCs were characterized by an immature phenotype. We systemically injected various populations of human UCB-derived MSCs into NOD/SCID mice after irradiation. The aim of our study was to evaluate the capacity of UCB-MSCs to promote the engraftment of hHSCs in these mice. However, our results showed that the ability of UCB-MSCs to promote engraftment is host dependent. To identify the
Acknowledgments
Y.S.Y. and Y.H.N. contributed equally to this work.
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Y.S.Y and Y.H.N contributed equally to this work.
Supported by a grant from the national R&D program for Cancer control, Ministry for Health, Welfare and Family affairs, Republic of Korea (Project No: 0720230) and by Samsung Biomedical Research Institute grant, #SBRI C-A7-201-3.