Transplantation Proceedings
Volume 42, Issue 6 , Pages 2036-2039 , July 2010

Rapid Quantitative Assessment of the Pig Pancreas Biopsy Predicts Islet Yield

  • T. Anazawa

      Affiliations

    • Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • A.N. Balamurugan

      Affiliations

    • Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, Minnesota, USA
    • Corresponding Author InformationAddress reprint requests to A.N. Balamurugan, Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA
    • ,
  • S. Matsumoto

      Affiliations

    • Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • S.A. LaFreniere

      Affiliations

    • Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • T.D. O'Brien

      Affiliations

    • Veterinary Population Medicine, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • D.E.R. Sutherland

      Affiliations

    • Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • B.J. Hering

      Affiliations

    • Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, Minnesota, USA

References 

  1. Hering BJ, Wijkstrom M, Graham ML, et al. Prolonged diabetes reversal after intraportal xenotransplantation of wild-type porcine islets in immunosuppressed nonhuman primates. Nat Med. 2006;12:301
  2. Hering BJ, Walawalkar N. Pig-to-nonhuman primate islet xenotransplantation. Transpl Immunol. 2009;21:81
  3. Dufrane D, d'Hoore W, Goebbels RM, et al. Parameters favouring successful adult pig islet isolations for xenotransplantation in pig-to-primate models. Xenotransplantation. 2006;13:204
  4. Kim H-I, Lee S-Y, Jin SM, et al. Parameters for successful pig islet isolation as deter- mined using 68 specific-pathogen-free miniature pigs. Xenotransplantation. 2009;16:11
  5. Krickhahn M, Bühler C, Meyer T, et al. The morphology of islets within the porcine donor pancreas determines the isolation result: successful isolation of pancreatic islets can now be achieved from young market pigs. Cell Transplant. 2002;11:827
  6. Gazda LS, Adkins H, Bailie JA, et al. The use of pancreas biopsy scoring provides reliable porcine islet yields while encapsulation permits the determination of microbiological safety. Cell Transplant. 2005;14:427
  7. Ferrer J, Scott WE, Weegman BP, et al. Pig pancreas anatomy: implications for pan- creas procurement, preservation, and islet isolation. Transplantation. 2008;86:1503
  8. Kirchhof N, Shibata S, Wijkstrom M, et al. Reversal of diabetes in non-immunosuppressed rhesus macaques by intraportal porcine islet xenografts precedes acute cellular rejection. Xenotransplantation. 2004;11:396
  9. Ricordi C, Gray DW, Hering BJ, et al. Islet isolation assessment in man and large animals. Acta Diabetol Lat. 1990;27:185

 Supported in part by grants from the Juvenile Diabetes Research Foundation (JDRF PPG #21-2006-881) and the National Institutes of Health (U19 AI067151).

PII: S0041-1345(10)00778-5

doi: 10.1016/j.transproceed.2010.05.113

Transplantation Proceedings
Volume 42, Issue 6 , Pages 2036-2039 , July 2010

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