Transplantation Proceedings
Volume 42, Issue 6 , Pages 2032-2035 , July 2010

Improved Method of Porcine Pancreas Procurement With Arterial Flush and Ductal Injection Enhances Islet Isolation Outcome

References 

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  2. Lakey JRT, Kneteman NM, Rajotte RV, et al. Effect of core pancreas temperature during cadaveric procurement on human islet isolation and functional viability. Transplantation. 2002;73:1106
  3. Wright MJ, Cavanagh TJ, Fetterhoff TJ, et al. Effect of blood content on porcine pancreatic dissociation and islet yield. Transplant Proc. 1994;26:3442
  4. Kirchhof N, Shibata S, Wijkstrom M, et al. Reversal of diabetes in non-immunosuppressed rhesus macaques by intraportal porcine islet xenografts precedes acute cellular rejection. Xenotransplantation. 2004;11:396
  5. Hering BJ, Wijkstrom M, Graham ML, et al. Prolonged diabetes reversal after intraportal xenotransplantation of wild-type porcine islets in immunosuppressed nonhuman primates. Nat Med. 2006;12:301
  6. Ricordi C, Gray DW, Hering BJ, et al. Islet isolation assessment in man and large animals. Acta Diabetol Lat. 1990;27:185
  7. Papas KK, Colton CK, Nelson RA, et al. Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice. Am J Transplant. 2007;7:707
  8. Papas KK, Pisania A, Wu H, et al. A stirred microchamber for oxygen consumption rate measurements with pancreatic islets. Biotechnol Bioeng. 2007;98:1071
  9. Prabhakaran S, Hering BJ. What strain of pig should be used?. Xenotransplantation. 2008;15:83
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 Supported in part by grants from the Juvenile Diabetes Research Foundation (JDRF PPG #21-2006-881) and the National Institutes of Health (U19 AI067151).

PII: S0041-1345(10)00775-X

doi: 10.1016/j.transproceed.2010.05.110

Transplantation Proceedings
Volume 42, Issue 6 , Pages 2032-2035 , July 2010