Transplantation Proceedings
Volume 42, Issue 6 , Pages 2024-2026 , July 2010

Improved Method of Human Islet Isolation for Young Donors

  • M. Shimoda

      Affiliations

    • Division of Cardiology, Department of Internal Medicine, Baylor University Medical Center, Baylor Heart and Vascular Institute, Dallas, Texas, USA
    • Baylor Research Institute Fort-Worth Campus, Islet Cell Laboratory, Texas, USA
    • ,
  • H. Noguchi

      Affiliations

    • Baylor Research Institute Fort-Worth Campus, Islet Cell Laboratory, Texas, USA
    • ,
  • B. Naziruddin

      Affiliations

    • Baylor Regional Transplant Institute, Dallas and Fort-Worth, Texas, USA
    • Institute of Biomedical Studies, Baylor University, Waco, Texas, USA
    • ,
  • Y. Fujita

      Affiliations

    • Baylor Research Institute Fort-Worth Campus, Islet Cell Laboratory, Texas, USA
    • ,
  • D. Chujo

      Affiliations

    • Baylor Institute for Immunology Research, Dallas, Texas, USA
    • ,
  • M. Takita

      Affiliations

    • Baylor Research Institute Fort-Worth Campus, Islet Cell Laboratory, Texas, USA
    • ,
  • H. Peng

      Affiliations

    • Institute of Biomedical Studies, Baylor University, Waco, Texas, USA
    • ,
  • Y. Tamura

      Affiliations

    • Baylor Regional Transplant Institute, Dallas and Fort-Worth, Texas, USA
    • ,
  • G.S. Olsen

      Affiliations

    • Baylor Regional Transplant Institute, Dallas and Fort-Worth, Texas, USA
    • ,
  • K. Sugimoto

      Affiliations

    • Baylor Research Institute Fort-Worth Campus, Islet Cell Laboratory, Texas, USA
    • ,
  • T. Itoh

      Affiliations

    • Baylor Research Institute Fort-Worth Campus, Islet Cell Laboratory, Texas, USA
    • ,
  • N. Onaca

      Affiliations

    • Baylor Regional Transplant Institute, Dallas and Fort-Worth, Texas, USA
    • ,
  • M.F. Levy

      Affiliations

    • Baylor Regional Transplant Institute, Dallas and Fort-Worth, Texas, USA
    • ,
  • P.A. Grayburn

      Affiliations

    • Division of Cardiology, Department of Internal Medicine, Baylor University Medical Center, Baylor Heart and Vascular Institute, Dallas, Texas, USA
    • ,
  • S. Matsumoto

      Affiliations

    • Baylor Research Institute Fort-Worth Campus, Islet Cell Laboratory, Texas, USA
    • Corresponding Author InformationAddress reprint requests to Shinichi Matsumoto, MD, PhD, Baylor Research Institute Fort Worth Campus, Islet Cell Laboratory, 1400 8th Ave, Fort Worth, TX 76104

References 

  1. Shapiro AM, Lakey JR, Ryan EA, et al. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med. 2000;343:230
  2. Robertson RP. Islet transplantation as a treatment for diabetes—a work in progress. N Engl J Med. 2004;350:694
  3. Matsumoto S, Okitsu T, Iwanaga Y, et al. Insulin independence after living-donor distal pancreatectomy and islet allotransplantation. Lancet. 2005;365:1642
  4. Matsumoto S, Okitsu T, Iwanaga Y, et al. Successful islet transplantation from nonheartbeating donor pancreata using modified Ricordi islet isolation method. Transplantation. 2006;82:460
  5. Ryan EA, Paty BW, Senior PA, et al. Five-year follow-up after clinical islet transplantation. Diabetes. 2005;54:2060
  6. Lakey JR, Warnock GL, Rajotte RV, et al. Variables in organ donors that affect the recovery of human islets of Langerhans. Transplantation. 1996;61:1047
  7. Hanley SC, Paraskevas S, Rosenberg L. Donor and isolation variables predicting human islet isolation success. Transplantation. 2008;85:950
  8. Socci C, Davalli AM, Vignali A, et al. A significant increase of islet yield by early injection of collagenase into the pancreatic duct of young donors. Transplantation. 1993;55:661
  9. Ricordi C. Islet transplantation: a brave new world. Diabetes. 2003;52:1595
  10. Ricordi C, Alejandro R, Zeng Y, et al. Human islet isolation and purification from pediatric-age donors. Transplant Proc. 1991;23:783
  11. Socci C, Davalli AM, Vignali A, et al. A significant increase of islet yield by early injection of collagenase into the pancreatic duct of young donors. Transplantation. 1993;55:661
  12. Balamurugan AN, Chang Y, Bertera S, et al. Suitability of human juvenile pancreatic islets for clinical use. Diabetologia. 2006;49:1845
  13. Matsumoto S, Noguchi H, Naziruddin B, et al. Improvement of pancreatic islet cell isolation for transplantation. Proc (Bayl Univ Med Cent). 2007;20:357
  14. Noguchi H, Ikemoto T, Naziruddin B, et al. Iodixanol-controlled density gradient during islet purification improves recovery rate in human islet isolation. Transplantation. 2009;87:1629
  15. Ikemoto T, Noguchi H, Shimoda M, et al. Islet cell transplantation for the treatment of type 1 diabetes in the USA. J Hepatobiliary Pancreat Surg. 2009;16:118
  16. Matsumoto S, Noguchi H, Hatanaka N, et al. Estimation of donor usability for islet transplantation in the United States with the Kyoto islet isolation method. Cell Transplant. 2009;18:549
  17. Buggenhout A, Hoang AD, Hut F, et al. Pediatric en bloc dual kidney–pancreas transplantation into an adult recipient: a simplified technique (Benefits of the en bloc kidney–pancreas transplantation technique in pediatric donors). Am J Transplant. 2004;4:663

 This study was partially supported by All Saints Health Foundation.

PII: S0041-1345(10)00759-1

doi: 10.1016/j.transproceed.2010.05.094

Transplantation Proceedings
Volume 42, Issue 6 , Pages 2024-2026 , July 2010

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