Transplantation Proceedings
Volume 42, Issue 6 , Pages 2016-2019 , July 2010

Persufflation Improves Pancreas Preservation When Compared With the Two-Layer Method

  • W.E. Scott III

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • T.D. O'Brien

      Affiliations

    • Department of Veterinary Population Medicine, University of Minnesota, Veterinary Diagnostic Laboratory, University of Minnesota, St Paul, Minnesota, USA
    • ,
  • J. Ferrer-Fabrega

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • E.S. Avgoustiniatos

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • B.P. Weegman

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • T. Anazawa

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • S. Matsumoto

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • V.A. Kirchner

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • M.D. Rizzari

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • Department of Surgery, University of Texas Southwestern, Dallas, Texas, USA
    • ,
  • M.P. Murtaugh

      Affiliations

    • Department of Veterinary and Biomedical Sciences, University of Minnesota, St Paul, Minnesota, USA
    • ,
  • T.M. Suszynski

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • T. Aasheim

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • L.S. Kidder

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • B.E. Hammer

      Affiliations

    • Department of Radiology, Center for Interdisciplinary Applications in Magnetic Resonance, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • S.G. Stone

      Affiliations

    • Giner Inc, Newton, Massachusetts, USA
    • ,
  • L.A. Tempelman

      Affiliations

    • Giner Inc, Newton, Massachusetts, USA
    • ,
  • D.E.R. Sutherland

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • B.J. Hering

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • ,
  • K.K. Papas

      Affiliations

    • Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
    • Corresponding Author InformationAddress reprint requests to Klearchos K. Papas, PhD, Schulze Diabetes Institute, University of Minnesota Medical School, Mayo Mail Code 195, 420 Delaware St SE, Minneapolis, MN 55455

References 

  1. Shapiro AM, Lakey JR, Ryan EA, et al. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med. 2000;343:289
  2. Shapiro AM, Ricordi C, Hering B. Edmonton's islet success has indeed been replicated elsewhere. Lancet. 2003;362:1242
  3. Shapiro AM, Lakey JR, Ryan EA, et al. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med. 2000;343:230
  4. Hering BJ, Kandaswamy R, Ansite JD, et al. Single donor marginal dose islet transplantation in patients with type 1 diabetes. JAMA. 2005;293:830
  5. Wang GL, Semenza GL. General involvement of hypoxia-inducible factor 1 in transcriptional response to hypoxia. Proc Natl Acad Sci USA. 1993;90:4304
  6. Dionne KE, Colton CK, Yarmush ML. Effect of hypoxia on insulin secretion by isolated rat and canine islets of Langerhans. Diabetes. 1993;42:12
  7. Moritz W, Meier F, Stroka DM, et al. Apoptosis in hypoxic human pancreatic islets correlates with HIF-1 alpha expression. FASEB J. 2002;16:745
  8. Bruick RK. Expression of the gene encoding the proapoptotic Nip3 protein is induced by hypoxia. Proc Natl Acad Sci U S A. 2000;97:9082
  9. Hering BJ, Matsumoto I, Sawada T, et al. Impact of two-layer pancreas preservation on islet isolation and transplantation. Transplantation. 2002;74:1813
  10. Avgoustiniatos ES, Hering BJ, Papas KK. The rat pancreas is not an appropriate model for testing the preservation of the human pancreas with the two-layer method. Transplantation. 2006;81:1471
  11. Papas KK, Hering BJ, Guenther L, et al. Pancreas oxygenation is limited during preservation with the two-layer method. Transplant Proc. 2005;38:3501
  12. Kin T, Mirbolooki M, Salehi P, et al. Islet isolation and transplantation outcomes of pancreas preserved with University of Wisconsin solution versus two-layer method using preoxygenated perfluorocarbon. Transplantation. 2006;82:1286
  13. Caballero-Corblan J, Eich T, Foss A, et al. No beneficial effect of the two layer method (PFCUW) compared with transportation in UW alone on the outcome of human islet isolation and transplantation: a report on 214 human pancreases. Am J Transplant. 2006;2(6 Suppl):340
  14. Caballero-Corblan J, Eich T, Lundgren T, et al. No beneficial effect of two-layer storage compared with UW-storage on human islet isolation and transplantation. Transplantation. 2007;84:864
  15. Ferrer J, Scott WE, Weegman BP, et al. Pig pancreas anatomy: implications for pancreas procurement, preservation, and islet isolation. Transplantation. 2008;86:1503
  16. Kuroda Y, Kawamura T, Suzuki Y, et al. A new, simple method for cold storage of the pancreas using perfluorochemical. Transplantation. 1988;46:457
  17. Papas KK, Colton CK, Nelson RA, et al. Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice. Am J Transplant. 2007;7:707

 Research funding provided by a grant from the National Center for Research Resources (U42 RR016598), National Institutes of Health (NIH), National Institute of Diabetes and Digestive and Kidney Diseases (R43 DK070400), NIH, the Schott Foundation, and the Carol Olson Memorial Diabetes Research Fund.

PII: S0041-1345(10)00757-8

doi: 10.1016/j.transproceed.2010.05.092

Transplantation Proceedings
Volume 42, Issue 6 , Pages 2016-2019 , July 2010

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