Pancreas Oxygen Persufflation Increases ATP Levels as Shown by Nuclear Magnetic Resonance

Scott, W.E.; Weegman, B.P.; Ferrer-Fabrega, J.; Stein, S.A.; Anazawa, T.; Kirchner, V.A.; Rizzari, M.D.; Stone, J.; Matsumoto, S.; Hammer, B.E.; Balamurugan, A.N.; Kidder, L.S.; Suszynski, T.M.; Avgoustiniatos, E.S.; Stone, S.G.; Tempelman, L.A.; Sutherland, D.E.R.; Hering, B.J.; Papas, K.K.

doi:10.1016/j.transproceed.2010.05.091

« Previous Next »Transplantation Proceedings
Volume 42, Issue 6 , Pages 2011-2015, July 2010

Pancreas Oxygen Persufflation Increases ATP Levels as Shown by Nuclear Magnetic Resonance

W.E. Scott III
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
B.P. Weegman
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
J. Ferrer-Fabrega
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
S.A. Stein
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
T. Anazawa
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
V.A. Kirchner
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
M.D. Rizzari
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
- Department of Surgery, University of Texas Southwestern, Dallas, Texas, USA
J. Stone
- Department of Surgery, University of Minnesota, Minneapolis, Minnesota, USA
S. Matsumoto
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
B.E. Hammer
- Department of Radiology, Center for Interdisciplinary Applications in Magnetic Resonance, University of Minnesota, Minneapolis, Minnesota, USA
A.N. Balamurugan
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
L.S. Kidder
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
T.M. Suszynski
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
E.S. Avgoustiniatos
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
S.G. Stone
- Giner Inc., Newton, Massachusetts
L.A. Tempelman
- Giner Inc., Newton, Massachusetts
D.E.R. Sutherland
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
B.J. Hering
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
K.K. Papas
- Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA
- Address reprint requests to Klearchos K. Papas, PhD, Schulze Diabetes Institute, University of Minnesota Medical School, Mayo Mail Code 195, 420 Delaware St SE, Minneapolis, MN 55455

Abstract

Background

Islet transplantation is a promising treatment for type 1 diabetes. Due to a shortage of suitable human pancreata, high cost, and the large dose of islets presently required for long-term diabetes reversal; it is important to maximize viable islet yield. Traditional methods of pancreas preservation have been identified as suboptimal due to insufficient oxygenation. Enhanced oxygen delivery is a key area of improvement. In this paper, we explored improved oxygen delivery by persufflation (PSF), ie, vascular gas perfusion.

Methods

Human pancreata were obtained from brain-dead donors. Porcine pancreata were procured by en bloc viscerectomy from heparinized donation after cardiac death donors and were either preserved by either two-layer method (TLM) or PSF. Following procurement, organs were transported to a 1.5-T magnetic resonance (MR) system for ³¹P nuclear magnetic resonance spectroscopy to investigate their bioenergetic status by measuring the ratio of adenosine triphosphate to inorganic phosphate (ATP:P_i) and for assessing PSF homogeneity by MRI.

Results

Human and porcine pancreata can be effectively preserved by PSF. MRI showed that pancreatic tissue was homogeneously filled with gas. TLM can effectively raise ATP:P_i levels in rat pancreata but not in larger porcine pancreata. ATP:P_i levels were almost undetectable in porcine organs preserved with TLM. When human or porcine organs were preserved by PSF, ATP:P_i was elevated to levels similar to those observed in rat pancreata.

Conclusion

The methods developed for human and porcine pancreas PSF homogeneously deliver oxygen throughout the organ. This elevates ATP levels during preservation and may improve islet isolation outcomes while enabling the use of marginal donors, thus expanding the usable donor pool.

To access this article, please choose from the options below

Supported by a grant from the National Center for Research Resources (U42 RR016598), National Institutes of Health (NIH), NIH National Institute of Diabetes and Digestive and Kidney Diseases (R43 DK070400), the Schott Foundation, and the Carol Olson Memorial Diabetes Research Fund.

PII: S0041-1345(10)00756-6

doi:10.1016/j.transproceed.2010.05.091

« Previous Next »Transplantation Proceedings
Volume 42, Issue 6 , Pages 2011-2015, July 2010

Access this article on SciVerse ScienceDirect