Role of Purines on Hepatic Ischemia–Reperfusion Lesions in Rabbit

Taha, M.O.; Simões, M.J.; Mendonça, M.V.; Lugo, F.R.C.; Carmo-Filho, R.; Lazarini, J.L.; Bessa, M.Z.; Marconi, T.D.; Magalhães, K.G.; Soares, J.H.; Tersariol, I.L.S.; Monteiro, H.P.; Oliveira, I.; Jurkiewicz, A.; Caricati-Neto, A.

doi:10.1016/j.transproceed.2009.01.057

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Volume 41, Issue 3 , Pages 807-811, April 2009

Role of Purines on Hepatic Ischemia–Reperfusion Lesions in Rabbit

M.O. Taha
- Department of Surgery, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil
- Address reprint request to Murched Omar Taha, MD/PhD, Professor of Department of Surgery – Federal University of São Paulo – Escola Paulista de Medicina, Rua Botucatu, 740, CEP 04023-900, São Paulo-SP, Brazil
M.J. Simões
- Department of Morphology, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil
M.V. Mendonça
- Medicine School of Federal University of Grande Dourados (UFGD), Grande Dourados–MS, Brazil
F.R.C. Lugo
- Medicine School of Federal University of Grande Dourados (UFGD), Grande Dourados–MS, Brazil
R. Carmo-Filho
- Medicine School of Federal University of Grande Dourados (UFGD), Grande Dourados–MS, Brazil
J.L. Lazarini Junior
- Department of Pharmacology, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil
M.Z. Bessa
- Department of Pharmacology, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil
T.D. Marconi
- Department of Pharmacology, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil
K.G. Magalhães
- Department of Pharmacology, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil
J.H. Soares
- Department of Surgery, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil
I.L.S. Tersariol
- University of Mogi das Cruzes, São Paulo, Brazil
H.P. Monteiro
- Department of Biochemistry, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil
I. Oliveira Jr
- Department of Morphology, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil
A. Jurkiewicz
- Department of Pharmacology, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil
A. Caricati-Neto
- Department of Pharmacology, Federal University of São Paulo, Escola Paulista de Medicina, São Paulo, Brazil

Abstract

In this work, we evaluate the effects of adenosine 5′ triphosphate (ATP) on hepatic lesions caused by ischemia/reperfusion (I/R) in liver rabbit. Rabbits were pretreated with ATP (15 mg/kg IV) or saline solution 0.9% (SS), before the hepatic I/R procedure. We evaluated the effects of ATP on hepatic injury before and after I/R. The warm hepatic I/R procedure caused profound acute liver injury, as indicated by elevated serum aspartate aminotransferase, alanine aminotransferase, and lactic dehydrogenase levels, as well as a high apoptotic cell count. All these changes were attenuate by ATP treatment before the hepatic I/R procedure. These results suggested that ATP exerted protective effects on hepatic I/R lesions in the rabbit. This ATP effect may be related to improved energy metabolism during reperfusion in ischemic livers protecting against functional damage of cellular and subcellular membranes during lipid peroxidation.

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Supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Coordenadoria de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil.

PII: S0041-1345(09)00118-3

doi:10.1016/j.transproceed.2009.01.057