Circulating Adhesion Molecules and Purine Nucleotides During Kidney Allograft Reperfusion

Domanski, L.; Pawlik, A.; Safranow, K.; Gryczman, M.; Sulikowski, T.; Jakubowska, K.; Olszewska, M.; Dziedziejko, V.; Ostrowski, M.; Chlubek, D.; Ciechanowski, K.

doi:10.1016/j.transproceed.2008.08.152

« Previous Next »Transplantation Proceedings
Volume 41, Issue 1 , Pages 40-43, January 2009

Circulating Adhesion Molecules and Purine Nucleotides During Kidney Allograft Reperfusion

L. Domanski
- Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland
A. Pawlik
- Department of Pharmacokinetics and Therapeutic Drug Monitoring, Pomeranian Medical University, Szczecin, Poland
- Address reprint requests to Dr Andrzej Pawlik, Department of Pharmacokinetics and Therapeutic Drug Monitoring, Powst. Wlkp. 72, 70-111, Szczecin, Poland
K. Safranow
- Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
M. Gryczman
- Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland
T. Sulikowski
- Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland
K. Jakubowska
- Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
M. Olszewska
- Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
V. Dziedziejko
- Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
M. Ostrowski
- Department of General and Transplantation Surgery, Pomeranian Medical University, Szczecin, Poland
D. Chlubek
- Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
K. Ciechanowski
- Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland

Abstract

The impairment of organ function due to ischemia-reperfusion injury is still an important problem in solid organ transplantation. Numerous experimental and clinical studies of native organs have shown that ischemia-reperfusion constitutes an acute inflammatory process involving cell surface adhesion molecule expression. These markers are crucial for the recruitment and infiltration of effector cells into the postischemic tissue. Purines released by the postischemic tissue as the products of the degradation of high-energy nucleotides can be regarded as markers of disturbed energy metabolism. The aim of this study was to examine the correlation between circulating adhesion molecules and purine metabolites in graft renal vein plasma during 49 cases of kidney reperfusion. E-selectin, ICAM-1, and VCAM-1 concentrations correlated positively with hypoxanthine concentrations during reperfusion, whereas the concentrations of ICAM-1 correlated negatively with xanthine concentrations. The results of the present study suggested that the concentrations of adhesion molecules in the renal vein during reperfusion correlated with purine metabolites, reflecting metabolic changes in renal tissue.