Improved Renal Function of Warm Ischemically Damaged Kidneys Using Polysol

Schreinemachers, M.C.J.M.; Doorschodt, B.M.; Florquin, S.; Idu, M.M.; Tolba, R.H.; van Gulik, T.M.

doi:10.1016/j.transproceed.2008.08.146

« Previous Next »Transplantation Proceedings
Volume 41, Issue 1 , Pages 32-35, January 2009

Improved Renal Function of Warm Ischemically Damaged Kidneys Using Polysol

M.C.J.M. Schreinemachers
- Surgical Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
B.M. Doorschodt
- Surgical Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
S. Florquin
- Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
M.M. Idu
- Department of Surgery, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
R.H. Tolba
- Institute for Laboratory Animal Science & Experimental Surgery, RTWH-Aachen University, Aachen, Germany
T.M. van Gulik
- Surgical Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
- Address reprint requests to: T.M. van Gulik, MD, Surgical Laboratory, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands

Abstract

Objective

We sought to assess the efficacy of POLYSOL, a low-viscosity, colloid-based organ preservation solution, for the preservation of warm ischemically damaged kidney grafts compared with histidine-tryptophane-ketoglutarate (HTK) solution.

Methods

Pigs (25–30 kg) underwent a left nephrectomy after clamping the renal vessels for 30 minutes. Kidney grafts washed out with Polysol (n = 6) or HTK (n = 6) were cold stored (CS) for 20 hours at 4°C. After the preservation period, the contralateral kidney was removed and the preserved kidney implanted heterotopically. Renal function was assessed daily for 7 days. Thereafter, animals were killed and the kidney grafts removed for histologic analysis.

Results

All animals survived for 7 days. All Polysol CS–preserved grafts showed immediate function, as demonstrated by urine production within 24 hours after reperfusion as compared with 3/6 grafts in the HTK CS group. Overall, the Polysol CS group showed improved renal function compared with HTK CS. Also, peak serum creatinine and blood urea values were lower in the Polysol CS group compared with HTK-preserved grafts. Histologic evaluation of warm ischemically damaged grafts showed less glomerular shrinking, less tubular damage, less edema, less inflammatory infiltration, and less necrosis in Polysol compared with HTK-preserved grafts.

Conclusion

Application of Polysol solution for washout and CS preservation of warm ischemically damaged kidney grafts resulted in improved renal function and structural integrity when compared with HTK.