Protective Effect of an Inhibitor of Interleukin-8 (Meraxin) From Ischemia and Reperfusion Injury in a Rat Model of Kidney Transplantation

Neri, F.; Puviani, L.; Tsivian, M.; Prezzi, D.; Pacilé, V.; Cavallari, G.; Bertelli, R.; Bianchi, E.; Piras, G.L.; Pariali, M.; Cavalieri, B.; Bertini, R.; Faenza, A.; Nardo, B.

doi:10.1016/j.transproceed.2007.05.018

« Previous Next »Transplantation Proceedings
Volume 39, Issue 6 , Pages 1771-1772, July 2007

Protective Effect of an Inhibitor of Interleukin-8 (Meraxin) From Ischemia and Reperfusion Injury in a Rat Model of Kidney Transplantation

F. Neri
- Department of Surgery and Transplantations, S. Orsola Hospital, University of Bologna, Bologna, Italy
L. Puviani
- Department of Surgery and Transplantations, S. Orsola Hospital, University of Bologna, Bologna, Italy
M. Tsivian
- Department of Surgery and Transplantations, S. Orsola Hospital, University of Bologna, Bologna, Italy
D. Prezzi
- Department of Surgery and Transplantations, S. Orsola Hospital, University of Bologna, Bologna, Italy
V. Pacilé
- Department of Surgery and Transplantations, S. Orsola Hospital, University of Bologna, Bologna, Italy
G. Cavallari
- Department of Surgery and Transplantations, S. Orsola Hospital, University of Bologna, Bologna, Italy
R. Bertelli
- Department of Surgery and Transplantations, S. Orsola Hospital, University of Bologna, Bologna, Italy
E. Bianchi
- Applied Biomedical Research Center (CRBa), S. Orsola Hospital, University of Bologna, Bologna, Italy
G.L. Piras
- Applied Biomedical Research Center (CRBa), S. Orsola Hospital, University of Bologna, Bologna, Italy
M. Pariali
- Applied Biomedical Research Center (CRBa), S. Orsola Hospital, University of Bologna, Bologna, Italy
B. Cavalieri
- Dompè SpA, L’Aquila, Italy.
R. Bertini
- Dompè SpA, L’Aquila, Italy.
A. Faenza
- Department of Surgery and Transplantations, S. Orsola Hospital, University of Bologna, Bologna, Italy
B. Nardo
- Department of Surgery and Transplantations, S. Orsola Hospital, University of Bologna, Bologna, Italy
- Address reprint requests to Bruno Nardo, MD, PhD, Department of Surgery and Transplantation, S. Orsola Hospital, Via Massarenti 9, 40138 Bologna, Italy.

Abstract

Introduction

Since the ischemia and reperfusion injury is one of the main causes of delayed graft function after transplantation, research efforts have focused on studying the molecules involved in this inflammatory process. The chemokine interleukin-8 (IL-8) seems to be the main one responsible through a chemoattractive action toward neutropils. Therefore, one of the strategies adopted to prevent this process is blocking the binding between IL-8 and its receptors. The aim of our study was to test the effect of meraxin, a new derivative from repertaxin, to protect the renal graft from ischemia and reperfusion injury.

Materials and methods

Eighty male syngenic rats were divided into four groups. The control group underwent only kidney transplantation, while the other groups were treated with meraxin at various dosages 2 hours before graft reperfusion. Blood and histological samples were taken at sacrifice 24 hours after transplantation.

Results

Creatinine was significantly lower in the group treated with the high dosage of meraxin. Histological observation of the grafted tissue showed instead only a mild and not significant neutrophilic infiltration, equal in each group.

Conclusions

Graft function was improved by the administration of meraxin at high dosage, but this effect did not seem to be connected to a reduction in inflammatory infiltration in the parechymal tissue. Maybe the cause is in the mechanisms of clotting activation, due to alteration of adhesion molecules and endothelial cells.