Molecular Markers in Stored Kidneys Using Perfluorocarbon-based Preservation Solution: Preliminary Results

Maluf, D.G.; Mas, V.R.; Yanek, K.; Stone, J.J.; Weis, R.; Massey, D.; Spiess, B.; Posner, M.P.; Fisher, R.A.

doi:10.1016/j.transproceed.2006.02.109

« Previous Next »Transplantation Proceedings
Volume 38, Issue 5 , Pages 1243-1246, June 2006

Molecular Markers in Stored Kidneys Using Perfluorocarbon-based Preservation Solution: Preliminary Results

D.G. Maluf
- Division of Transplant, Department of Surgery, Virginia Commonwealth University, Richmond, Virginia
- Address reprint requests to Dr Daniel G Maluf, Assistant Professor of Surgery, Transplantation Division, Department of Surgery, Virginia Commonwealth University, WEST HOSPITAL 9TH FL, PO Box: 980057, Richmond, VA 23298-0248.
V.R. Mas
- Division of Transplant, Department of Surgery, Virginia Commonwealth University, Richmond, Virginia
- Department of Pathology, Virginia Commonwealth University, Richmond, Virginia
K. Yanek
- Division of Transplant, Department of Surgery, Virginia Commonwealth University, Richmond, Virginia
J.J. Stone
- Division of Transplant, Department of Surgery, Virginia Commonwealth University, Richmond, Virginia
R. Weis
- Department of Anesthesiology, Virginia Commonwealth University, Richmond, Virginia
- VCU Reanimation Engineering Shock Center VCURES, Virginia Commonwealth University, Richmond, Virginia.
D. Massey
- Department of Pathology, Virginia Commonwealth University, Richmond, Virginia
B. Spiess
- Department of Anesthesiology, Virginia Commonwealth University, Richmond, Virginia
- VCU Reanimation Engineering Shock Center VCURES, Virginia Commonwealth University, Richmond, Virginia.
M.P. Posner
- Division of Transplant, Department of Surgery, Virginia Commonwealth University, Richmond, Virginia
R.A. Fisher
- Division of Transplant, Department of Surgery, Virginia Commonwealth University, Richmond, Virginia

Abstract

Background

Delayed graft function (DGF) is a problem in kidney transplantation and cold ischemia has been identified as a risk factor. Perfluorocarbons (PFC) have an enhanced ability to dissolve and release oxygen. We evaluated histologically and a number of molecular changes induced by ischemia in stored kidneys with University of Wisconsin (UW) and PFC-based preservation solutions (PFC-UW).

Materials and methods

ACI rats were used as kidney donors. UW (control group) or PFC-UW (study group) preservation solutions were used for kidney perfusion. All kidneys were stored at 4°C for 12, 24, and 36 hours. After this time, intragraft histologic evaluation as well as mRNA HO-1 and iNOS levels were also analyzed.

Results

In the kidneys stored at 24 hours, mRNA HO-1 levels were elevated in the study group when compared with the control and mRNA iNOS was decreased.

Conclusion

We observed overexpression of HO-1 and underexpression of iNOS in the kidney tissue stored with PFC-UW solution at 24 hours. These preliminary data suggest that increasing oxygen delivery by PFC added to the perfusion solution triggers cytoprotective mechanism in kidney transplantation.