Protective Role of 3-Nitro-N-Methyl-Salicylamide on Isolated Rat Heart During 4 Hours of Cold Storage and Reperfusion

Zhang, S.; Yang, J.H.; Yu, F.; Zhao, J.; Jiang, P.; Chang, L.; Tang, C.; Xu, J.

doi:10.1016/j.transproceed.2006.02.061

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Volume 38, Issue 5 , Pages 1247-1252, June 2006

Protective Role of 3-Nitro-N-Methyl-Salicylamide on Isolated Rat Heart During 4 Hours of Cold Storage and Reperfusion

S. Zhang
- National Laboratory of Biomacromolecule, Center for Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, China
J.H. Yang
- Department of Physiopathology, Institute of Medicine, University of Beijing, Beijing, China.
F. Yu
- Department of Physiopathology, Institute of Medicine, University of Beijing, Beijing, China.
J. Zhao
- National Laboratory of Biomacromolecule, Center for Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, China
- Department of Physiopathology, Institute of Medicine, University of Beijing, Beijing, China.
P. Jiang
- Department of Physiopathology, Institute of Medicine, University of Beijing, Beijing, China.
L. Chang
- National Laboratory of Biomacromolecule, Center for Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, China
- Department of Physiopathology, Institute of Medicine, University of Beijing, Beijing, China.
C. Tang
- Department of Physiopathology, Institute of Medicine, University of Beijing, Beijing, China.
J. Xu
- National Laboratory of Biomacromolecule, Center for Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, China
- Address reprint requests to J. Xu, National Laboratory of Biomacromolecule, Center for Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China.

Abstract

Objective

Cardiac ischemia/reperfusion (I/R) injury, a necessary consequence of transplantation, is probably related to the formation of reactive oxygen species (ROS). The ROS burst within the first moments of reperfusion is associated with injury, continuously generate O₂⁻ at about 3% to 5% of total O₂ consumption owing to electron leak by mitochondrial oxidoreductases, especially complexes I and III. 3-nitro-N-methyl-salicylamide (NNMS) displays inhibitory effects on succinate-cytochrome C reductase, but also reduces effects on creation of O₂⁻ radical and H₂O₂ by isolated rat mitochondria. Presumably NNMS inhibits electron leakage from the mitochondrial respiratory chain. We investigated effect of NNMS on heart protection after hypothermic ischemia.

Methods

A Langendorff-prepared rat heart model was employed after the heart had been preserved for 4 hours under hypothermic conditions of ischemia with subsequent reperfusion/rewarming for 60 minutes.

Results

The group of hearts treated with NNMS showed increased recovery of heart function compared with a group of mEC. The lactate dehydrogenase (LDH) activity in coronary flow (CF) by hearts treated with NNMS was lower than that with mECs, as was the content of malonedialdehyde (MDA) and conjugated diene (CD).