Transplantation Proceedings
Volume 38, Issue 1 , Pages 16-19 , January 2006

Expression of Stem Cell Markers on Mononuclear Cells Derived From Heparinized Cadaveric Organ Donors Before and After Disconnection From the Respirator

  • B. Machaliński

      Affiliations

    • Department of General Pathology, Pomeranian Medical University, Szczecin, Poland
    • Corresponding Author InformationAddress reprint requests to Bogusław Machaliński, Department of General Pathology, Pomeranian Medical University, Al. Powstańców Wlkp. 72, 70-111 Szczecin, Poland
    • ,
  • E. Paczkowska

      Affiliations

    • Department of General Pathology, Pomeranian Medical University, Szczecin, Poland
    • ,
  • M. Hałasa

      Affiliations

    • Department of General Pathology, Pomeranian Medical University, Szczecin, Poland
    • ,
  • K. Pabisiak

      Affiliations

    • Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland
    • ,
  • M. Walczak

      Affiliations

    • Department of Pediatrics, Pomeranian Medical University, Szczecin, Poland
    • ,
  • J. Sieńko

      Affiliations

    • Department of General and Transplant Surgery, Pomeranian Medical University, Szczecin, Poland
    • ,
  • W. Kozik

      Affiliations

    • Department of Anatomy, Pomeranian Medical University, Szczecin, Poland
    • ,
  • M. Ostrowski

      Affiliations

    • Department of General and Transplant Surgery, Pomeranian Medical University, Szczecin, Poland
    • ,
  • A. Syrenicz

      Affiliations

    • Department of General Pathology, Pomeranian Medical University, Szczecin, Poland
    • ,
  • T. Sulikowski

      Affiliations

    • Department of General and Transplant Surgery, Pomeranian Medical University, Szczecin, Poland
    • ,
  • A. Machalińska

      Affiliations

    • Department of General Pathology, Pomeranian Medical University, Szczecin, Poland

References 

  1. Machaliński B , Kijowski J , Marlicz W , et al.   Heparinized cadaveric organ donors (HCOD)—a potential source of hematopoietic cells for transplantation and gene therapy . Transplantation . 2001;71:1003
  2. Marlicz W , Paczkowski M , Kijowski J , et al.   Isolation of hematopoietic stem cells from heparinized cadaveric multiple organ donorspotential clinical implications . Transplant Proc . 1999;31:2099
  3. Machaliński B , Paczkowski M , Kawa M , et al.   An optimisation of isolation of early hematopoietic cells from heparinized cadaveric organ donors . Transplant Proc . 2003;35:3096
  4. Machaliński B . Isolation of haematopoietic cells from heparinized cadaveric organ donors for transplantation purposes . Ann Acad Med Stetin . 1999;45(suppl 60):1
  5. Machaliński B , Baśkiewicz-Masiuk M , Pabisiak K , et al.   The evaluation of apoptosis and reactive oxygen species (ROS) in hematopoietic cells derived from heparinized cadaveric organ donors (HCOD) short-term stored at 4°C . Transplant Proc . 2005;37:2281
  6. Civin CI , Strauss LC , Brovall C , et al.   Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells . J Immunol . 1984;133:157
  7. Kvalheim G . Clinical use of enriched CD34+ cells . Blood Ther Med . 2002;2:57
  8. Majka M , Ratajczak J , Machaliński B , et al.   Expression, regulation, and function of AC133, a putative cell surface marker of primitive human haematopoietic cells . Folia Histochem Cytobiol . 2000;38:53
  9. Ratajczak J , Machaliński B , Majka M , et al.   Evidence that human haematopoietic stem cells (HSC) do not residue within the CD34+KIT− cell population . Ann Transplant . 1999;4:22
  10. Forraz N , Pettengell R , McGuckin CP . Characterisation of a lineage-negative stem-progenitor cell population optimised for ex vivo expansion and enriched for LTC-IC . Stem Cells . 2004;22:100
  11. Yin AH , Miraglia S , Zanjani ED , et al.   AC133, a novel marker for human hematopoietic stem and progenitor cells . Blood . 1997;90:5002
  12. Ratajczak J , Reca R , Kucia M , et al.   Mobilisation studies in mice deficient in either C3 or C3a receptor (C3aR) reveal a novel role for complement in retention of hematopoietic stem/progenitor cells in bone marrow . Blood . 2004;103:2071
  13. Tavor S , Petit I , Porozov S , et al.   CXCR4 regulates migration and development of human acute myelogenous leukemia stem cells in transplanted NOD/SCID mice . Cancer Res . 2004;64:2817
  14. Ratajczak MZ , Pletcher CH , Marlicz W , et al.   CD34+, kit+, rhodamine123(low) phenotype identifies a marrow cell population highly enriched for human hematopoietic stem cells . Leukemia . 1998;12:942
  15. Wojakowski W , Tendera M , Michalowska A , et al.   Mobilisation of CD34/CXCR4+, CD34/CD117+, c-met+ stem cells, and mononuclear cells expressing early cardiac, muscle, and endothelial markers into peripheral blood in patients with acute myocardial infarction . Circulation . 2004;110:3213
  16. Kucia M , Dawn B , Hunt G , et al.   Cells expressing early cardiac markers reside in the bone marrow and are mobilized into the peripheral blood after myocardial infarction . Circ Res . 2004;95:1191
  17. Kollet O , Shivtiel S , Chen YQ , et al.   HGF, SDF-1, and MMP-9 are involved in stress-induced human CD34+ stem cell recruitment to the liver . J Clin Invest . 2003;112:160
  18. Peled A , Petit I , Kollet O , et al.   Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4 . Science . 1999;283:845
  19. Yuan R , Astle CM , Chen J , et al.   Genetic regulation of hematopoietic stem cell exhaustion during development and growth . Exp Hematol . 2005;33:243
  20. Kamminga LM , van Os R , Ausema A , et al.   Impaired hematopoietic stem cell functioning after serial transplantation and during normal ageing . Stem Cells . 2005;23:82

 This work was supported by State Committee for Scientific Research grant PBZ-KBN-083/P05/2002 from 2003 to 2005 and European Union grant QLK3-CT-2002-30307 to B.M.

PII: S0041-1345(05)01548-4

doi: 10.1016/j.transproceed.2005.12.070

Transplantation Proceedings
Volume 38, Issue 1 , Pages 16-19 , January 2006

Article Tools

* Image Usage & Resolution