Transplantation Proceedings
Volume 36, Issue 3 , Pages 605-606 , April 2004

An alternative and simple method to consistently prepare viable isolated human islets for clinical transplantation

  • M Bugliani

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • R Lupi

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • S Del Guerra

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • U Boggi

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • L Marselli

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • S Sbrana

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • F Vistoli

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • S Torri

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • M Del Chiaro

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • S Signori

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • F Filipponi

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • S Del Prato

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • M Campa

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • V Corsini

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • A Campatelli

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • F Di Candio

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • F Mosca

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • ,
  • P Marchetti

      Affiliations

    • Department of Endocrinology and Metabolism, Metabolic Unit, Department of Oncology, Transplant Unit, and Department of Bioscience, University of Pisa, Pisa, Italy
    • Corresponding Author InformationAddress reprint requests to Piero Marchetti, MD, Dipartimento di Endocrinologia e Metabolismo, Ortopedia e Traumatologia, Medicina del Lavoro (Sezione Metabolismo), via Paradisa 2, Ospedale Cisanello, 56100 Pisa - Italy tel +39.050.995101 fax +39.050.541521

References 

  1. Ricordi C, Lacy PE, Finke EH, et al.  Automated method for isolation of human pancreatic islets. Diabetes. 1988;37:413
  2. Scharp DW, Lacy PE, Santiago JV, et al.  Insulin indipendence after islet transplantation into type I diabetic patient. Diabetes. 1990;39:515
  3. Shapiro AM, Lakey JR, Ryan EA, et al.  Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med. 2000;343:230
  4. Kenmochi T, Miyamoto M, Une S, et al.  Improved quality and yield of islets isolated from human pancreata using a two-step digestion method. Pancreas. 2000;20:184
  5. Marchetti P, Lupi R, Federici M, et al.  Insulin secretory function is impaired in isolated human islets carrying the Gly(972) → Arg IRS-1 polymorphism. Diabetes. 2002;51:1419

☆ This work was supported by Italian Ministry of Health and MIUR (2002-2003).

PII: S0041-1345(04)00191-5

doi: 10.1016/j.transproceed.2004.02.047

Transplantation Proceedings
Volume 36, Issue 3 , Pages 605-606 , April 2004

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