Physiological studies on vasoactive intestinal peptide in rat small bowel isografts

Tomita, R; Fujisaki, S; Park, E; Kimizuka, K; Ikede, T; Koshinaga, T; Shibata, M; Tanjoh, K

doi:10.1016/j.transproceed.2003.12.011

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Volume 36, Issue 2 , Pages 367-369, March 2004

Physiological studies on vasoactive intestinal peptide in rat small bowel isografts

R Tomita
- First Department of Surgery, Nihon University School of Medicine (R.T., S.F., E.P., K.K., T.I., T.K., M.S., K.T.), Tokyo, Japan
- Department of Surgery, Nippon Dental University School of Dentistry at Tokyo (R.T.), Tokyo, Japan
- Address reprint requests to Prof Ryouichi Tomita, MD, PhD, Department of Surgery, Nippon Dental University Hospital, 2-3-16 Fujimi Chiyoda-ku, Tokyo 102-8158, Japan.
S Fujisaki
- First Department of Surgery, Nihon University School of Medicine (R.T., S.F., E.P., K.K., T.I., T.K., M.S., K.T.), Tokyo, Japan
E Park
- First Department of Surgery, Nihon University School of Medicine (R.T., S.F., E.P., K.K., T.I., T.K., M.S., K.T.), Tokyo, Japan
K Kimizuka
- First Department of Surgery, Nihon University School of Medicine (R.T., S.F., E.P., K.K., T.I., T.K., M.S., K.T.), Tokyo, Japan
T Ikede
- First Department of Surgery, Nihon University School of Medicine (R.T., S.F., E.P., K.K., T.I., T.K., M.S., K.T.), Tokyo, Japan
T Koshinaga
- First Department of Surgery, Nihon University School of Medicine (R.T., S.F., E.P., K.K., T.I., T.K., M.S., K.T.), Tokyo, Japan
M Shibata
- First Department of Surgery, Nihon University School of Medicine (R.T., S.F., E.P., K.K., T.I., T.K., M.S., K.T.), Tokyo, Japan
K Tanjoh
- First Department of Surgery, Nihon University School of Medicine (R.T., S.F., E.P., K.K., T.I., T.K., M.S., K.T.), Tokyo, Japan

Abstract

Background

Vasoactive intestinal peptide (VIP) is released by stimulation of nonadrenergic noncholinergic (NANC) inhibitory nerves. In order to evaluate the function of VIP in jejunal isografts, we examined the enteric nerve responses in isografted rat jejunum compared with normal jejunum.

Methods

Orthotopic entire small bowel transplantation (SBT) with portocaval drainage was performed from Lewis rats to Lewis rats. Grafted tissue specimens were obtained 130 days after SBT (n = 8). As controls, normal segments of the jejunum were obtained from nontransplanted Lewis rats (n = 20). A mechanograph was used to evaluate in vitro jejunal responses to electrical field stimulation (EFS) of the adrenergic and cholinergic nerves before and after treatment with various autonomic nerve blockers and VIP.

Results

The isografted jejunum was more strongly innervated by excitatory nerves, especially NANC excitatory nerves, than the normal jejunum (P < .05). VIP mediated relaxation reactions of NANC inhibitory nerves in the normal but to a lesser extent in the isografted jejunum (P < .05).

Conclusions

The increased NANC excitatory nerves and the decreased effects of VIP in mediating NANC inhibitory nerves may largely relate to the peristaltic abnormalities seen in the isografted LEW rat jejunum.